Monoclonal Mouse anti-human CD3, CD16 and CD56 is recommended for use in flow cytometry for identification of Natural Killer (CD3-CD16+CD56+) and a subpopulation of T lymphocytes (CD3+).The CD3 monoclonal antibody is directed against the CD3- antigen (T3-antigen), which is expressed on human T lymphocytes. The monoclonal antibody reacts with 80-90% human peripheral T lymphocytes and medullary thymocytes. The monoclonal antibody does not react with B-cells, monocytes, granulocytes and platelets. The monoclonal antibody is mitogenic for resting T lymphocytes and it blocks the cytolytic activity of CTL clones. The CD16 molecule has been described as the low affinity Fc receptor (FcRIII) for complexed IgG which may exist either as a transmembranous form or as glycosyl phosphatidylinositol form. It is expressed on NK cells, granulocytes (PMN) and macrophages. The GPI-linked form is also expressed on neutrophils.Regarding CD56, expression of neural cell adhesion molecules (N-CAM) provides neurons with a means of attaching to and interacting with other cells and the extracellular matrix. Alternative splicing of N-CAM mRNA results in several N-CAM isoforms. The three major isoforms are referred to as 180 kD, 140 kD, and 120 kD. Individual isoforms undergo various post-translational modifications, including the addition of polysialic acid residues to embryonic forms. The highly polysialylated forms of N-CAM present in embryos and neonates are lost during maturation and replaced by less-polysialic acid-laden mature forms of N-CAM. The functional significance of alternative forms of N-CAM remains to be fully elucidated.
CD3 FITC/CD16+56 PE CE-IVD