FLUOROCHROMES: PACIFIC BLUE, FITC, PE, PE-DYLIGHT 594, APC, PE-CY5, PE-CY7, PERCP, PERCP-CY5.5, AL. FLUOR 700, CF-BLUE, OC515, APC-C750.
The monoclonal antibody is directed against the CD4-antigen (T4-antigen),which is expressed on human peripheral T lymphocytes and 80% of thymocytes. The monoclonal antibody reacts on a low level with human monocytes and macrophages. The monoclonal antibody does not react with B-cells ,granulocytes and thrombocytes.Normal B lymphocytes, monocytes or granulocytes do not express surface CD4 antigen although cytoplasmic expression has been observed in monocytes/macrophages. The CD4 positive T lymphocyte subpopulation has been characterised functionally as comprising helper cells active in amplification of immune responses.